All functions

JPP_update()

Select alternate m_score column in JPP data and avert user

MSstats_data

Testing dataset in MSstats format.

OpenSWATH_data data

Testing dataset from OpenSWATH.

Spyogenes

S.pyogenes example data.

Study_design

A table containing the meta-data defining the study design of the OpenSWATH data.

add_genesymbol()

Adds gene symbols to a table

assess_decoy_rate()

Assess decoy rate in data

assess_fdr_byrun()

Assess assay, peptide and protein level FDR by run (for each MS_injection separately) in OpenSWATH output table

assess_fdr_overall()

Assess overall FDR in annotated OpenSWATH/pyProphet output table in dependence of m_score cutoff

convert4MSstats()

Get data ready for use by MSstats.

convert4PECA()

Convert table into the format for ROPECA

convert4aLFQ()

Convert table into the format expected by aLFQ.

convert4mapDIA()

Convert table into the format for mapDIA

convert4pythonscript()

Convert data into the format for running a python script.

convert_protein_ids()

Convert protein ids

count_analytes()

Counts the analytes across the different injections

disaggregate()

Transforms the SWATH data from a peptide- to a transition-level table.

filter_all_peptides()

Select all proteins that are supported by peptides.

filter_mscore()

Filter OpenSWATH output table based on mscore.

filter_mscore_condition()

Filter OpenSWATH output table according to mscore and conditions.

filter_mscore_fdr()

Filter annotated OpenSWATH/pyProphet output table to achieve a high FDR quality data matrix with controlled overall protein FDR and quantitative values for all peptides mapping to these high-confidence proteins (up to a desired overall peptide level FDR quality).

filter_mscore_freqobs()

Filter OpenSWATH output table according to mscore.

filter_on_max_peptides()

Filter only for the highest intense peptides

filter_on_min_peptides()

Filter openSWATH output for proteins that are identified by a minimum of n independent peptides.

filter_proteotypic_peptides()

Filter for proteins that are supported by proteotypic peptides.

import_data()

Transforms the column names from a data frame to the required format.

load_mart()

Establish connection to biomaRt database

mscore4assayfdr()

Find m_score cutoff to reach a desired FDR on assay level (over the entire OpenSWATH/pyProphet output table)

mscore4pepfdr()

Find m_score cutoff to reach a desired FDR on peptide level (over the entire OpenSWATH/pyProphet output table)

mscore4protfdr()

Find m_score cutoff to reach a desired FDR on protein level (over the entire OpenSWATH/pyProphet output table)

plot(<fdr_cube>)

S3 plot function for FDR assessment result arrays

plot(<fdr_table>)

S3 plot function for results of class "fdr_table" as produced by e.g. the function assess_fdr_overall()

plot_correlation_between_samples()

Plots the correlation between injections.

plot_variation()

Plots the coefficient of variation for different replicates.

plot_variation_vs_total()

Plots the total variation versus variation within replicates

reduce_OpenSWATH_output()

Reduce columns of OpenSWATH data

removeDecoyProteins()

Removes decoy proteins from the protein group label

rmDecoyProt()

Subfunction to remove decoys

sample_annotation()

Annotate the SWATH data with the sample information

transform_MSstats_OpenSWATH()

Transforms column names to OpenSWATH column names

unifyProteinGroupLabels()

Unify the protein group labels.

validate_columns()

Validate columns for a data.frame

write_matrix_peptides()

Writes out an overview matrix of peptides mapping to a FDR quality controlled protein master list at controlled global peptide FDR quality.

write_matrix_proteins()

Writes out an overview matrix of summed signals per protein identifier (lines) over run_id(columns).