All functions |
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Select alternate m_score column in JPP data and avert user |
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Testing dataset in MSstats format. |
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Testing dataset from OpenSWATH. |
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S.pyogenes example data. |
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A table containing the meta-data defining the study design of the OpenSWATH data. |
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Adds gene symbols to a table |
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Assess decoy rate in data |
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Assess assay, peptide and protein level FDR by run (for each MS_injection separately) in OpenSWATH output table |
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Assess overall FDR in annotated OpenSWATH/pyProphet output table in dependence of m_score cutoff |
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Get data ready for use by MSstats. |
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Convert table into the format for ROPECA |
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Convert table into the format expected by aLFQ. |
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Convert table into the format for mapDIA |
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Convert data into the format for running a python script. |
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Convert protein ids |
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Counts the analytes across the different injections |
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Transforms the SWATH data from a peptide- to a transition-level table. |
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Select all proteins that are supported by peptides. |
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Filter OpenSWATH output table based on mscore. |
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Filter OpenSWATH output table according to mscore and conditions. |
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Filter annotated OpenSWATH/pyProphet output table to achieve a high FDR quality data matrix with controlled overall protein FDR and quantitative values for all peptides mapping to these high-confidence proteins (up to a desired overall peptide level FDR quality). |
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Filter OpenSWATH output table according to mscore. |
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Filter only for the highest intense peptides |
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Filter openSWATH output for proteins that are identified by a minimum of n independent peptides. |
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Filter for proteins that are supported by proteotypic peptides. |
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Transforms the column names from a data frame to the required format. |
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Establish connection to biomaRt database |
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Find m_score cutoff to reach a desired FDR on assay level (over the entire OpenSWATH/pyProphet output table) |
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Find m_score cutoff to reach a desired FDR on peptide level (over the entire OpenSWATH/pyProphet output table) |
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Find m_score cutoff to reach a desired FDR on protein level (over the entire OpenSWATH/pyProphet output table) |
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S3 plot function for FDR assessment result arrays |
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S3 plot function for results of class "fdr_table" as produced by e.g. the function assess_fdr_overall() |
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Plots the correlation between injections. |
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Plots the coefficient of variation for different replicates. |
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Plots the total variation versus variation within replicates |
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Reduce columns of OpenSWATH data |
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Removes decoy proteins from the protein group label |
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Subfunction to remove decoys |
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Annotate the SWATH data with the sample information |
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Transforms column names to OpenSWATH column names |
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Unify the protein group labels. |
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Validate columns for a data.frame |
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Writes out an overview matrix of peptides mapping to a FDR quality controlled protein master list at controlled global peptide FDR quality. |
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Writes out an overview matrix of summed signals per protein identifier (lines) over run_id(columns). |